Born in Ghent, Zeger Vandersteene received soprano voice training at the Schola Cantorum of the Sint-Baafs Cathedral. His four-year training in Gregorian Chants has exerted a strong influence throughout his career. He continued his studies with great masters in Austria, Italy, the Netherlands and France. He prepared for his debut performances with S. Cape, R. Clemencic and N. Harnoncourt (music of the Middle Ages, the Renaissance and the Baroque).

He subsequently perfected his tenor voice and interpreted many lyric tenor roles : Steuerman (Der Fliegende Holländer), Aegist (Elektra), Jaquino (Fidelio), Ernesto (Don Pasquale), Les Pécheurs de Perles and Lakmé. He premiered five contemporary operas, notably in Munich, Berlin and Venice. He has sung in many countries with renown conductors such as Sir J. Pritchard, M. Janovski, R. Frühbeck de Burgos, E. Inbal, M. Soustrot, H. Vonk and Sir C. Mackerras, and has made serveral recordings for a variety of labels: Harmonia Mundi, Erato, Teldec, RGIP, among others.

In addition to his many master classes in Mexico, Japan and Germany, Zeger Vandersteene has taught at the Ghent Conservatory, where he now is an honorary professor.